ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (7): 1068-1074.doi: 10.11843/j.issn.0366-6964.2014.07.006

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Genetic Structure and Phylogenetic Evolution Analysis Based on Cytb Gene Polymorphism of Tibetan Mastiff

YANG Xue-jiao,REN Zhan-jun*,ZHANG Cheng-dong,BAI Kang   

  1. (College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China)
  • Received:2014-01-23 Online:2014-07-23 Published:2014-07-23

Abstract:

In order to provide reference for resource protection,rational development and utilization of Tibetan Mastiff,70 samples from Tibet and Gansu were studied.Genetic structure and phylogenetic evolution analysis were explored using sequencing method and bioinformatics softwares,based on mitochondrial Cytb gene.A total of 6 haplotypes were detected and 8 polymorphic loci accounting for 0.7% of the analyzed sites were found.The two populations had similar genetic diversity,the haplotype diversity(Hd),nucleotide diversity(Pi),the average number of nucleotide difference(K)of them were 0.595,0.002 37,2.703 and 0.674,0.002 51,2.857,respectively.The gene flow between the two populations was strong and variation mainly was from within populations.Infered from the haplotype network and phylogenetic tree,Tibetan Mastiff had single maternal origin which evolved into 3 branches.

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